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OGT’s hybridization-based targeted sequencing

June 15, 2017Oxford Gene Technology has made several advances in hybridization-based target enrichment protocols that enable researchers to prepare samples for sequencing in one day. Researchers can access the high-quality results of hybridization-based targeted sequencing with a speed comparable to amplicon-based approaches, according to a company statement.

OGT’s hybridization-based targeted sequencing delivers coverage uniformity, enabling researchers to detect low frequency variants consistently down to one percent variant allele frequency at a read depth of >1,000x. The company’s latest protocol developments enable even more researchers to quickly and precisely focus in on regions relevant to their research.

The hybridization assay enhancements improve speed and enable lower input levels of DNA to be used. The company has optimized its enzymes and buffers to allow pooling of library preparation steps, which make it possible for users to complete the enrichment protocol in less time. This includes a short enzymatic fragmentation step, combined end-repair and adaptor ligation steps, and an optimized hybridization of 30 minutes for good quality DNA samples. OGT’s NGS panel optimization yields good data with as little as 10 ng of starting material, according to the company.

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