You have no items to compare.
Product Guides
Compare Products
Our online product guides make it easy to view and compare instruments and software systems feature by feature. Each listing includes an Add to Compare Products link. Click this link to compare two or more products. At the bottom of these instructions, you'll see a list of your choices. When you click the Compare button, the products you are comparing will show up side by side in a new window. You can print and/or save the comparisons you create.
| Name of instrument | Ion Personal Genome Machine (PGM) |
| Name of model/Model has been upgraded | 508-U001/no |
| Country where designed/Manufactured/FDA-cleared or approved | U.S./Singapore/pre-IDE submission |
| First year sold in U.S./Outside U.S./First year installed | 2010/2010/2010 |
| Dimensions in inches (H × W × D)/Footprint of all instrumentation and computing hardware | 21 × 24 × 20/5 square feet |
| Equipment supplied with system/Automation for library prep | Dell Precision T7500 Server/yes |
| Necessary equipment not included with system and additional cost | compressed nitrogen cylinder (for pressurizing the Ion PGM Sequencer): ~$70, Ion OneTouch System (automated template preparation): ~$14,500, Elga Purelab Flex 3 water purification system (fresh 18MΩ water) ~$5,000 |
| Bioinformatics tools provided/For use by biologist or bioinformatician | Ion Reporter (biologist), Torrent Suite software (biologist), Torrent Circuit (biologist) |
| Supplied with UPS/Entire workflow can occur in same lab | no/yes |
| Clean room requirements/Electrical connection | clean room always recommended for pre-amp steps/100–240 VAC, 50–60 Hz, 9 VA |
| List price/Total list price for equipment needed to perform simplest and fastest workflow from amplif through variant calling (not typically found in lab) | $49,500/$49,500 (Ion PGM Sequencer), $16,500 (Torrent Server), $14,500 (Ion OneTouch System), $5,700 (Elga Purelab water purification system, plus nitrogen gas) |
| Purchase options | purchase, reagent rental, or lease (financing available) |
| Warranties offered | 1-year included, extended warranty available |
| Training included/Total time for standard install and basic training | yes/install: 1 day; training: 2 days |
| Training location/Follow-up training available | on site and off site/yes, extra charge |
| Instrument core performance: | |
| Maximum No. of libraries amplified in single amplif event | 384 samples by employing custom bar-coding |
| Read length/Percent bases >Q30 | 200+ bp, 300+ bp* (Q3 of 2012), 400+ bp (Q4 of 2012)*/75* |
| Paired-end capability/tag lengths/spans | yes/2 × 100 bp/~100–400* |
| Fragment/tag lengths/spans | yes/200 bp, 300 bp (Q3 of 2012)*, 400 bp (Q4 of 2012)*/~100–400* |
| Mate-pair/tag lengths/spans | yes/60 bp/~2–10 kb |
| Single-end/tag lengths/spans | yes/200 bp, 300 bp (Q3 of 2012)*, 400 bp (Q4 of 2012)*/~100–400* |
| RNA sequencing/tag lengths/spans | yes/200 bp, 300 bp (Q3 of 2012)*, 400 bp (Q4 of 2012)*/~100–400* |
| ChIP sequencing/tag lengths/spans | yes/200 bp, 300 bp (Q3 of 2012)*, 400 bp (Q4 of 2012)*/~100–400* |
| Bisulfite sequencing/tag lengths/spans | yes, customer demonstrated/~100–400* |
| Output per run | 20 Mb (Ion 314 chip, 200 bp kit); 200 Mb (Ion 316 chip, 200 bp kit); ≤1 Gb (Ion 318 chip, 200 bp kit) |
| Total time from library construction to variant calling to achieve output per run/Technical bench time/Bioinformatics time | 7–14 hours (35 to 300 bp*)/2 hours (library, template, PGM, Ion Chip preparation)/≤30 minutes |
| Sample preparation: | |
| Total time for generating standard gDNA library | <2 hours |
| • Paired-end | ~3 hours |
| • Fragment | <2 hours |
| • Mate-pair | ~18 hours |
| • Single-end | <2 hours |
| • RNA sequencing | ~6 hours |
| • ChIP sequencing | customer demonstrated |
| • Bisulfite sequencing | customer demonstrated |
| Hands-on time each: | |
| • Paired-end | ~15 minutes (IonXpress Fragment), ~10 minutes (Ion AmpliSeq 2.0) |
| • Fragment | ~15 minutes (IonXpress Fragment), ~10 minutes (Ion AmpliSeq 2.0) |
| • Mate-pair | ~6 hours |
| • Single-end | ~15 minutes (IonXpress Fragment), ~10 minutes (Ion AmpliSeq 2.0) |
| • RNA sequencing | ~40 minutes (whole transciptome library, small RNA library); ~55 minutes for small RNA library with enrichment |
| • ChIP sequencing | customer demonstrated |
| • Bisulfite sequencing | customer demonstrated |
| Equipment required for library construction | if automation required, recommend AB Library Builder system |
| Reagents and controls: | |
| Cost per run | $349 (Ion 314 chip), $549 (Ion 316 chip), $749 (Ion 318 chip) |
| Cost per sample* | 30x coverage, 200 bp: $88.06 (Ion 318 chip), $213.75 (Ion 316 chip), $750.83 (Ion 314 chip) |
| Reagent tracking method on instrument | bar-code reader |
| Information contained in tracking method | external bar-code reader for sample and reagent tracking information |
| Reagent shipping conditions/Storage conditions | -20°C, 4°C, and ambient/-20°C, 4°C, and ambient |
| Shelf life of amplification and sequencing reagents | ≤12 months |
| Controls introduced during creation of library/Sequencing control avail. | yes/yes |
| Capable of complete walkaway automation for amp, seq, var calling | yes |
| Remote system monitoring | yes |
| Instrument control software and devices to start run/for data analysis | touchscreen user interface/Torrent Suite |
| Total time required for setup of amplification, sequencing, and variant calling steps | 30 minutes |
| Maximum No. of libraries sequenced in a single run | 384 |
| Types of maintenance plans available | AB Assurance, AB Complete, AB Maintenance Plus, AB Maintenance Service |
| No. of field apps scientists and engineers based in U.S. | field applications scientists: 40; engineers: 90 |
| Weekly maintenance required/monthly/pre-run | weekly: chlorite cleaning; monthly: none; pre-run: daily wash solution cleaning |
| System offers secondary analysis software developed by instrument vendor | yes |
| Variant report generated directly on instrument | yes |
| Third-party analysis software available | yes (DNAStar SoftGenetics, Partek, Avadis NGS, CLC Bio, Bio Team) |
| Ability of software to detect mutations | substitutions, indels, copy number changes |
| Total No. of peer-reviewed publications for this platform | 22 |
| Published applications in pathology-related research | 8 |
| Fastest published turnaround time from sample to analyzed result | 62 hours |
| Lowest published variant sensitivity level | 0.25–5.5 percent (Yang, et al.) |
| Distinguishing features of sequencer (supplied by company) | flexibility to meet a range of applications, budgets, project sizes, and input DNA amounts (as low as 10 ng of FFPE-derived sample), by employing scalable semiconductor technology with the Ion 3 series chips, coupled with highly multiplexed amplicon generation using Ion AmpliSeq technology; delivers fast sequencing run times, while maintaining high accuracy, i.e., 30 minutes for 35 bp, 2 hours for 200+ bp, with concomitant Q30 (>99.9 percent) accuracy; high-throughput, low-cost capital acquisition; no optics, lasers, or cameras enables low maintanance and run costs |
| *inclusive of sample prep, for multiple configurations, using 1 Mb and 30x average coverage per sample | *expected performance; product not officially released, information subject to change without notice |
| Note: a dash in lieu of an answer means company did not answer question or question is not applicable |
© 2010 CAP TODAY. All Rights Reserved.