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Roche Diagnostics Corp., GS FLX+ (Sequencing, September 2012)

Roche Diagnostics Corp., GS FLX+ (Sequencing, September 2012)

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Contact Information

Roche Diagnostics Corp.
Clotilde Teiling
clotilde.teiling@roche.com
9115 Hague Road
Indianapolis, IN 46256
317-521-2000


Name of instrument GS FLX+**
Name of model/Model has been upgraded GS FLX+/yes
Country where designed/Manufactured/FDA-cleared or approved U.S./U.S./—
First year sold in U.S./Outside U.S./First year installed 2005/2006/2005
Dimensions in inches (H × W × D)/Footprint of all instrumentation and computing hardware 51 × 30 × 36/50 square feet
Equipment supplied with system/Automation for library prep sequencing instrument, attendant computer, control and analysis software/yes
Necessary equipment not included with system and additional cost data analysis workstation, QIAGEN TissueLyser II, bead counter, microplate centrifuge, thermal cycler, hood/—
Bioinformatics tools provided/For use by biologist or bioinformatician GS Amplicon Variant Analyzer, GS De Novo Assembler, GS Reference Mapper (all are GUI-based)/biologist
Supplied with UPS/Entire workflow can occur in same lab yes/yes
Clean room requirements/Electrical connection none/120 V or 230 V, 50 or 60 Hz
List price/Total list price for equipment needed to perform simplest and fastest workflow from amplif through variant calling (not typically found in lab) $425,000/—
Purchase options purchase, lease, or rent
Warranties offered 1-year manufacturing, extended service contracts
Training included/Total time for standard install and basic training yes/5 days
Training location/Follow-up training available on site and off site/yes (no additional charge)
Instrument core performance:
Maximum No. of libraries amplified in single amplif event 192+
Read length/Percent bases >Q30 up to 1,000 bp (700 bp average mode)/88
Paired-end capability/tag lengths/spans yes/180 average/3 kb, 8 kb, 20 kb, 40 kb
Fragment/tag lengths/spans yes/full read length/—
Mate-pair/tag lengths/spans yes/180 average/3 kb, 8 kb, 20 kb, 40 kb
Single-end/tag lengths/spans yes/full read length/—
RNA sequencing/tag lengths/spans yes/full read length/—
ChIP sequencing/tag lengths/spans yes/full read length/—
Bisulfite sequencing/tag lengths/spans yes/full read length/—
Output per run ~700 Mb (1,000,000+ reads)
Total time from library construction to variant calling to achieve output per run/Technical bench time/Bioinformatics time 36 hours/6.5 hours/3 hours (full titanium run)
Sample preparation:
Total time for generating standard gDNA library 3 hours
• Paired-end 36 hours
• Fragment 1.5 hours
• Mate-pair 36 hours
• Single-end 3 hours
• RNA sequencing 36 hours
• ChIP sequencing 1.5 hours
• Bisulfite sequencing 1.5 hours
Hands-on time each: 2 hours
• Paired-end 8 hours
• Fragment .75 hour
• Mate-pair 8 hours
• Single-end 2 hours
• RNA sequencing 8 hours
• ChIP sequencing .75 hour
• Bisulfite sequencing .75 hour
Equipment required for library construction supplied with kit
Reagents and controls:
Cost per run $4,490 (approximate)
Cost per sample* depends on experimental design
Reagent tracking method on instrument bar-coded reagents
Information contained in tracking method part and lot numbers, expiration date
Reagent shipping conditions/Storage conditions ambient and dry ice/ambient, 4°C, -20°C
Shelf life of amplification and sequencing reagents 12–18 months
Controls introduced during creation of library/Sequencing control avail. no/yes
Capable of complete walkaway automation for amp, seq, var calling no
Remote system monitoring yes
Instrument control software and devices to start run/for data analysis GUI-based, on instrument/GUI-based, off instrument
Total time required for setup of amplification, sequencing, and variant calling steps 6 hours
Maximum No. of libraries sequenced in a single run 192+
Types of maintenance plans available full coverage
No. of field apps scientists and engineers based in U.S. 259
Weekly maintenance required/monthly/pre-run monthly: maintenance wash; pre-run: fully integrated wash with run protocol
System offers secondary analysis software developed by instrument vendor yes
Variant report generated directly on instrument no
Third-party analysis software available yes (multi-vendor capability)
Ability of software to detect mutations substitutions, indels, copy number changes
Total No. of peer-reviewed publications for this platform 1,500+
Published applications in pathology-related research 300+
Fastest published turnaround time from sample to analyzed result 24 hours
Lowest published variant sensitivity level 0.01%
Distinguishing features of sequencer (supplied by company) up to 1,000 base pair reads (comparable to Sanger); long reads allow accurate linkage of variants and transcript assemblies; GS FLX system technology available in benchtop format with the GS Junior system
*inclusive of sample prep, for multiple configurations, using 1 Mb and 30x average coverage per sample
Note: a dash in lieu of an answer means company did not answer question or question is not applicable **For life science research only. Not for use in diagnostic procedures.