You have no items to compare.
| Name of instrument | GS FLX+** |
| Name of model/Model has been upgraded | GS FLX+/yes |
| Country where designed/Manufactured/FDA-cleared or approved | U.S./U.S./— |
| First year sold in U.S./Outside U.S./First year installed | 2005/2006/2005 |
| Dimensions in inches (H × W × D)/Footprint of all instrumentation and computing hardware | 51 × 30 × 36/50 square feet |
| Equipment supplied with system/Automation for library prep | sequencing instrument, attendant computer, control and analysis software/yes |
| Necessary equipment not included with system and additional cost | data analysis workstation, QIAGEN TissueLyser II, bead counter, microplate centrifuge, thermal cycler, hood/— |
| Bioinformatics tools provided/For use by biologist or bioinformatician | GS Amplicon Variant Analyzer, GS De Novo Assembler, GS Reference Mapper (all are GUI-based)/biologist |
| Supplied with UPS/Entire workflow can occur in same lab | yes/yes |
| Clean room requirements/Electrical connection | none/120 V or 230 V, 50 or 60 Hz |
| List price/Total list price for equipment needed to perform simplest and fastest workflow from amplif through variant calling (not typically found in lab) | $425,000/— |
| Purchase options | purchase, lease, or rent |
| Warranties offered | 1-year manufacturing, extended service contracts |
| Training included/Total time for standard install and basic training | yes/5 days |
| Training location/Follow-up training available | on site and off site/yes (no additional charge) |
| Instrument core performance: | |
| Maximum No. of libraries amplified in single amplif event | 192+ |
| Read length/Percent bases >Q30 | up to 1,000 bp (700 bp average mode)/88 |
| Paired-end capability/tag lengths/spans | yes/180 average/3 kb, 8 kb, 20 kb, 40 kb |
| Fragment/tag lengths/spans | yes/full read length/— |
| Mate-pair/tag lengths/spans | yes/180 average/3 kb, 8 kb, 20 kb, 40 kb |
| Single-end/tag lengths/spans | yes/full read length/— |
| RNA sequencing/tag lengths/spans | yes/full read length/— |
| ChIP sequencing/tag lengths/spans | yes/full read length/— |
| Bisulfite sequencing/tag lengths/spans | yes/full read length/— |
| Output per run | ~700 Mb (1,000,000+ reads) |
| Total time from library construction to variant calling to achieve output per run/Technical bench time/Bioinformatics time | 36 hours/6.5 hours/3 hours (full titanium run) |
| Sample preparation: | |
| Total time for generating standard gDNA library | 3 hours |
| • Paired-end | 36 hours |
| • Fragment | 1.5 hours |
| • Mate-pair | 36 hours |
| • Single-end | 3 hours |
| • RNA sequencing | 36 hours |
| • ChIP sequencing | 1.5 hours |
| • Bisulfite sequencing | 1.5 hours |
| Hands-on time each: | 2 hours |
| • Paired-end | 8 hours |
| • Fragment | .75 hour |
| • Mate-pair | 8 hours |
| • Single-end | 2 hours |
| • RNA sequencing | 8 hours |
| • ChIP sequencing | .75 hour |
| • Bisulfite sequencing | .75 hour |
| Equipment required for library construction | supplied with kit |
| Reagents and controls: | |
| Cost per run | $4,490 (approximate) |
| Cost per sample* | depends on experimental design |
| Reagent tracking method on instrument | bar-coded reagents |
| Information contained in tracking method | part and lot numbers, expiration date |
| Reagent shipping conditions/Storage conditions | ambient and dry ice/ambient, 4°C, -20°C |
| Shelf life of amplification and sequencing reagents | 12–18 months |
| Controls introduced during creation of library/Sequencing control avail. | no/yes |
| Capable of complete walkaway automation for amp, seq, var calling | no |
| Remote system monitoring | yes |
| Instrument control software and devices to start run/for data analysis | GUI-based, on instrument/GUI-based, off instrument |
| Total time required for setup of amplification, sequencing, and variant calling steps | 6 hours |
| Maximum No. of libraries sequenced in a single run | 192+ |
| Types of maintenance plans available | full coverage |
| No. of field apps scientists and engineers based in U.S. | 259 |
| Weekly maintenance required/monthly/pre-run | monthly: maintenance wash; pre-run: fully integrated wash with run protocol |
| System offers secondary analysis software developed by instrument vendor | yes |
| Variant report generated directly on instrument | no |
| Third-party analysis software available | yes (multi-vendor capability) |
| Ability of software to detect mutations | substitutions, indels, copy number changes |
| Total No. of peer-reviewed publications for this platform | 1,500+ |
| Published applications in pathology-related research | 300+ |
| Fastest published turnaround time from sample to analyzed result | 24 hours |
| Lowest published variant sensitivity level | 0.01% |
| Distinguishing features of sequencer (supplied by company) | up to 1,000 base pair reads (comparable to Sanger); long reads allow accurate linkage of variants and transcript assemblies; GS FLX system technology available in benchtop format with the GS Junior system |
| *inclusive of sample prep, for multiple configurations, using 1 Mb and 30x average coverage per sample | |
| Note: a dash in lieu of an answer means company did not answer question or question is not applicable | **For life science research only. Not for use in diagnostic procedures. |