September 2025—New England Biolabs launched the NEBNext Low-bias Small RNA library prep kit, designed to minimize biased representation of small RNA species in sequencing data. A novel splint adaptor increases the diversity of interactions, facilitating ligation and increasing sensitivity, with a streamlined, simplified protocol. As a result, researchers can analyze all RNA species present in biologically relevant samples. Standard and 2’-O-methylated samples can also be processed using the same protocol, with multiplexing enabled through up to 480 compatible unique dual index primer pairs (available separately). Total workflow time is approximately 3.5 hours and the shelf life is 18 months.
New England Biolabs, 800-632-5227