CAP TODAY Pathology/Laboratory Medicine/Laboratory Management
December 2020—PCR Biosystems has introduced its NGSBIO Library Quant Kit for Illumina. The kit contains all the components necessary for accurate and sensitive quantification of DNA libraries prior to next-generation sequencing with Illumina platforms. The kit contains five DNA standards, primers specific to the p5 and p7 Illumina adapter sequences, a library dilution buffer, and qPCRBIO SyGreen Mix or qPCRBIO SyGreen Blue Mix. The blue qPCR mix contains a nonreactive dye to improve reaction mix visibility and is useful when using small reaction volumes or white plates.
PCR Biosystems provides a free online calculation tool to help scientists analyze results generated with the NGSBIO Library Quant Kit.
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