CAP TODAY Pathology/Laboratory Medicine/Laboratory Management
Beckman Coulter Life Sciences’ new reagent kits for intracellular staining minimize sample manipulation for a streamlined workflow. Developed for use on Beckman Coulter’s Gallios and Navios series flow cytometers and compatible with all Beckman fluorochromes, the kits enable a clear focus on the cytoplasmic and nuclear compartments of the cell.
PerFix-Expose (phospho-epitope exposure kit) helps scientists identify and explore complex intracellular signaling pathways disrupted by cancer and other diseases. The kit allows fluorochrome-coupled antibodies and other probes to enter cells via their nuclei and other cell compartments, where they bind to proteins relevant to signaling pathways that have been disrupted by disease.
With PerFix-nc (no centrifugation), researchers can perform concomitant surface and intracellular staining for flow cytometry to take advantage of a new approach that provides easy access to the inside of human leukocytes.
PerFix-nc kit quality is characterized by mean fluorescence intensities and consistent performance in repeatability studies, as demonstrated by levels of cell recovery. The kit offers a leukocyte permeabilization method followed by an optimized fixation step in a process that involves only 15 minutes of hands-on user time.
Beckman Coulter Life Sciences, 800-742-2345