March 2014—Enzo Life Sciences’ Histamine ELISA kit provides a quantitative detection method for measuring histamine in human, rat, mouse, and canine samples. Because of histamine’s potent role in the immune response, it is present in a transitory state in the body and can only be measured within minutes of release. The half-life of histamine in a biological system is four minutes before its conversion to n-methyl histamine. N-methyl histamine is a major product of histamine metabolism, is present in urine, and has a longer half-life than histamine. This assay reacts equally to n-methyl histamine and histamine, making it an excellent choice for measuring histamine levels in urine, according to a company statement.
The assays measure as little as 0.03 ng/mL of histamine in serum, plasma, urine, and tissue culture media and provide fully quantitative results that surpass semiquantitative Western blot analysis.
The kits can analyze up to 40 samples in duplicate in less than two hours and are supplied with an easy-to-follow protocol, pre-coated microtiter plate, and liquid color-coded reagents.
Enzo Life Sciences, 800-942-0430