Webinars and Sponsored Roundtables — Register Now

Wednesday, September 30, 2026. 1 PM-1:30 PM ET
Roundtable presenters John Longshore, PhD, Head of Scientific Affairs, Global Oncology Diagnostics, AstraZeneca, and Flora Berisha, MS, Executive Director, Global Head of Diagnostic Partnering and Development, Johnson & Johnson Innovative Medicine, and Mark D. Ewalt, MD, Associate Medical Director for Laboratory Operations, Diagnostic Molecular Pathology, Molecular Diagnostics Service, Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, and Isabel Preeshagul, DO, MBS, Thoracic Medical Oncologist, Memorial Sloan Kettering Cancer Center

CAP TODAY does not endorse any of the products or services named within. The roundtable is made possible by a special educational grant from Pillar Biosciences.

Moderated by: Bob McGonnagle, Publisher, CAP TODAY

Thursday, October 1, 2026 1 PM-2 PM ET
This presentation will explore the value of in-house CGP, offer insights into the benefits and drawbacks of incorporation, and provide information about important practical factors when considering in-house implementation of CGP.

Webinar presenter Allison M. Cushman-Vokoun, MD, PhD, FCAP, Medical Director, Molecular Diagnostics and Personalized Medicine Laboratory, Director, Division of Diagnostic Molecular Pathology and Human Genetics, Henry F. Krous Professor of Pathology, University of Nebraska Medical Center

CAP TODAY does not endorse any of the products or services named within. The webinar is made possible by a special educational grant from Illumina.

Moderated by: Bob McGonnagle, Publisher, CAP TODAY

Tuesday, October 20, 2026 11 AM-12 PM ET
Hear experts review the biological and clinical significance of the HER2 expression continuum in breast tissue, providing a clearer understanding of how these variations might impact diagnosis, and discuss the emerging importance of documenting HER2-low and HER2-ultralow categories using a validated IHC assay.

Webinar presenters Keith Wharton, MD, PhD, Global Medical Affairs Leader–Pathology, Roche Diagnostics Solutions, and Hannah Y. Wen, MD, PhD, Director, Breast Pathology Fellowship, Associate Team Leader, Breast Pathology Team, Attending Pathologist, Memorial Sloan Kettering Cancer Center

CAP TODAY does not endorse any of the products or services named within. The webinar is made possible by a special educational grant from Roche.

Moderated by: Bob McGonnagle, Publisher, CAP TODAY

Tuesday, November 3, 2026 1 PM-2 PM ET
Hear an expert discuss the challenges of detecting NRG1 fusions and how RNA-based testing can support more comprehensive fusion identification in clinical practice.

Webinar presenter Benjamin Weinberg, MD, Associate Professor of Medicine and Attending Physician
specializing in gastrointestinal medical oncology

CAP TODAY does not endorse any of the products or services named within. The webinar is made possible by a special educational grant from Diaceutics.

Moderated by: Bob McGonnagle, Publisher, CAP TODAY

Subspecialties

Interactive Product Guides

Product Spotlight

Illumina

MiSeq i100

Company

Illumina

Name of system

MiSeq i100

Email

info@illumina.com

City, State

San Diego, CA

Phone

858-202-4500  

Website

http://www.illumina.com

System application/FDA cleared or approved

research use/not required

Country where designed/Manufactured

U.S./Singapore

First year sold in U.S./First year installed in U.S.

2025/2025

System sold internationally

yes (worldwide)

Dimensions of sequencer (H × W × D)/Footprint of sequencer

25.6 × 15.8 × 17.6 in./1.9 sq. ft.

Accessory equipment supplied with sequencer at no cost

—

Type of computer supplied with sequencer

computer for analysis and operations combined

Analysis options provided with sequencer

onboard, cloud based

Where library preparation is performed

wet bench

Bioinformatics tools provided with sequencer

DRAGEN Bio-IT, BaseSpace Sequence Hub, Illumina Connected Analytics

Sequencer supplied with UPS (uninterruptible power supply)

no

Electrical connection required for sequencer

100–240 at VAC 50/60 Hz, 300 W, single phase

List price of entire sequencer and necessary components

$49, 900

Purchase options

purchase, lease, reagent rental

Warranties offered

—

Training included/Total time for basic training per operator

no/2 days

Training location/Follow-up training available

at vendor office or customer site/yes (extra charge)

Maximum No. of samples amplified in a single amplification event

384 samples

Read length/Percent bases >Q30

up to 2 × 300 bp/85% (for 2 × 300 bp)

Paired-end capability/Tag lengths/Spans

yes/up to 2 × 500 bp/950 bp

Fragment/Tag lengths/Spans

yes/up to 2 × 300 bp/—

Mate pair/Tag lengths/Spans

yes/up to 2 × 150 bp/—

Single end/Tag lengths/Spans

yes/up to 1 × 300 bp/—

RNA sequencing/Tag lengths/Spans

yes/up to 2 × 300 bp/—

ChIP sequencing/Tag lengths/Spans

yes/up to 2 × 300 bp/—

Bisulfite sequencing/Tag lengths/Spans

yes/up to 2 × 300 bp/—

Maximum No. of reads or fragments sequenced per single-end run

25 million

Maximum No. of reads or fragments sequenced per paired-end run

50 million

Total No. of nucleotides (bases) sequenced per run

25 Gb

Wet lab bench time for sequencing preparation

10 minutes

Sequencing run time

~4–24 hours

Total time for generating standard gDNA library

<3.5 hours (with Illumina DNA Prep)

• Paired end

<3.5 hours (with Illumina DNA Prep)

• Fragment

<7 hours (with AmpliSeq for Illumina); <6.5 hours with Illumina DNA Prep with Enrichment)

• Mate pair

—

• Single end

<1 day (with TruSeq Small RNA)

• RNA sequencing

<9 hours

• Bisulfite sequencing

—

• Hands-on time for paired end

<1.5 hours (with Illumina DNA Prep)

• Hands-on time for fragment

<1.5 hours (with AmpliSeq for Illumina); <2 hours (with Illumina DNA Prep with Enrichment)

• Hands-on time for mate pair

—

• Hands-on time for single end

<4 hours (with TruSeq Small RNA)

• Hands-on time for RNA sequencing

<3 hours

• Hands-on time for ChIP sequencing

—

• Hands-on time for bisulfite sequencing

—

Library preparation integrated in system as standard offering

no

Cost of sequencing reagents per run

$420–$1,700

Reagent tracking method

RFID

• Type of reagent information tracked

serial No., expiration date, lot and part Nos., No. of cycles

Shipping conditions for amplification/sequencing reagents

room temperature/room temperature

Storage conditions for amplification/sequencing reagents

room temperature/room temperature

Shelf life of amplification/sequencing reagents

guaranteed 3 months/guaranteed 3 months

System requires a control sample on sequencing run 

optional

• Company offers a sequencing control

yes (additional charge)

Sequencing system control software and devices to start run/for data analysis

MiSeq i100 Control Software/BaseSpace Sequence Hub, DRAGEN secondary analysis

Complete walkaway automation for amplification, sequencing, and variant calling

yes

Remote system monitoring

yes

Total time required for setup of amplification, sequencing, and variant calling steps

10 minutes

Maximum No. of libraries sequenced in a single run

384 samples

System includes secondary data-analysis software developed by company

yes (DRAGEN, BaseSpace Sequence Hub)

System includes post-sequencing data-analysis software

yes (DRAGEN)

Mutations detectable via data-analysis software

substitutions, indels, copy number changes

System can generate a variant report

yes

Types of maintenance plans available/mean time between failures

parts only, bronze, silver, gold, Dx, dedicated on-site/—

No. of field application scientists and engineers based in U.S.

>500

• Weekly maintenance required

none

• Monthly maintenance required

none

• Pre-run maintenance required

none

Distinguishing features of NGS system (supplied by company)

room temperature shipping and storage of sequencing consumables; automated onboard flow-cell denaturation, onboard cluster generation, no post-run washing; simplified data analysis with access to preconfigured DRAGEN pipelines onboard or in the cloud, minimizing the need for bioinformatics expertise

More products
in this guide

MiSeq i100 Plus
NextSeq 550Dx
NextSeq 1000; NextSeq 2000
NovaSeq 6000Dx
NovaSeq X; NovaSeq X Plus
Ion GeneStudio S5 System
Ion Torrent Genexus Integrated Sequencer; Ion Torrent Genexus Dx Integrated Sequencer